SARS-CoV-2 (COVID-19) Spike P681R Antibody [7E3C5] (Delta Variant), IgG2, Unconjugated, Mouse, Monoclonal

Article Name:	SARS-CoV-2 (COVID-19) Spike P681R Antibody [7E3C5] (Delta Variant), IgG2, Unconjugated, Mouse, Monoclonal
Biozol Catalog Number:	PRS-PM-9680
Supplier Catalog Number:	PM-9680
Alternative Catalog Number:	PRS-PM-9680-0.02,PRS-PM-9680-0.1
Manufacturer:	ProSci
Host:	Mouse
Category:	Antikörper
Application:	ELISA, IHC-P
Species Reactivity:	Virus
Immunogen:	Anti-SARS-CoV-2 Spike 681R (Delta Variant) antibody (PM-9680) was raised against a peptide corresponding to 12 amino acids near the cleavage site of SARS-CoV-2 Delta variant Spike protein. The immunogen is located within 650-700 amino acids of SARS-CoV-2 Delta variant Spike protein.
Conjugation:	Unconjugated
Alternative Names:	Anti-SARS-CoV-2 Spike P681R (Delta Variant) antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Delta variant, Spike protein, Surface Glycoprotein, covid-19, sars-cov-2

Clonality:	Monoclonal
Concentration:	1 mg/mL
Clone Designation:	[7E3C5]
Isotype:	IgG2
NCBI:	43740568
UniProt:	P0DTC2
Buffer:	SARS-CoV-2 Spike P681R (Delta Variant) antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	IHC: 20 µg/mL. Antibody validated: IHC in human samples. Anti-SARS-CoV-2 Spike P681R (Delta Variant) antibody specifically detects SARS-CoV-2 Delta Variant Spike S1 protein, but not SARS-CoV-2 WT and other variant Spike S1 protein by ELISA. All other applications and species not yet tested.

	Figure 2 SARS-Cov-2 Spike P681R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 &956,g/mL, incubated at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, PM-9680, 1 &956,g/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.
	Figure 3 ELISA Validation of Delta Variant Spike S1 Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein Coating Antigen: SARS-CoV-2 spike S1 proteins, WT, Alpha and Delta variants, 1 &956,g/mL, incubated at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, PM-9680, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 Spike P681R (Delta Variant) antibody specifically detects spike S1 protein of Delta variant, but not WT and Alpha variant.
	Figure 4 ELISA Validation of Delta Variant Spike S1 Antibodies with Mutant and WT Peptide Coating Antigen: SARS-CoV-2 spike S1 peptides: WT (9091P), Alpha variant (9359P), and Delta variant (9673P), 1 &956,g/mL, incubated at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 delta spike P681R antibody, PM-9680, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 Spike P681R (Delta Variant) antibody detects Delta variant spike S1 peptide (681R, 9673P), but not WT peptide (681P, 9091P) and Alpha variant spike S1 peptide (681H, 9359P).
	Figure 1 Immunohistochemistry Validation of Spike in Delta Variant COVID-19 Patient Lung Tissue Immunohistochemical analysis of paraffin-embedded patient lung tissue using anti-Spike P681R antibody (PM-9680) at 20 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in the Delta variant COVID-19 patient lung, but not in WT COVID-19 patient lung.