PD1 Antibody [7H6], IgG1, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: PD1 Antibody [7H6], IgG1, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: PRS-RF16003
• Supplier Catalog Number: RF16003
• Alternative Catalog Number: PRS-RF16003-0.02,PRS-RF16003-0.1
• Manufacturer: ProSci
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FC, ICC, IF, IHC-P, WB
• Species Reactivity: Human
• Immunogen: PD-1 antibody was raised against the extracellular domain of human PD-1.
• Conjugation: Unconjugated
• Alternative Names: PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/mL
• Clone Designation: [7H6]
• Molecular Weight: Predicted: 32 kDa Observed: 38 kDa
• Isotype: IgG1
• NCBI: 5133
• UniProt: Q15116
• Buffer: PD-1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: PD-1 antibody can be used for detection of PD-1 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples, Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.

Images

Flow cytometry analysis of PD-1 overexpressing 293 cells using RF16003 at 1 &956,g/ml. Blue: untransfected cells, Yellow: PD-1 transfected cells.

Western blot analysis of PD-1 in overexpressing 293 cells using RF16001, RF16002, and RF16003 antibody at 1, 0.5, and 0.25 &956,g/ml, respectively.

Titration curve analysis of PD-1 mAbs to detect recombinant PD-1 in ELISA with RF16001, RF16002, RF16003, RF16004, and RF16005 abs at decreasing concentrations.

Immunocytochemistry of PD-1 in transfected 293 cells with PD-1 antibody at 5 &956,g/mL. Lower left: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 5 &956,g/mL.

Immunofluorescence of PD-1 in transfected 293 cells with PD-1 antibody at 5 &956,g/mL. Lower left: Immunofluorescence in transfected 293 cells with control mouse IgG antibody at 5 &956,g/mL.Red: PD1 Antibody [7H6] (RF16003) Blue: DAPI staining

Immunohistochemistry of PD-1 in (A) human tonsil tissue, (B) human lymph node tissue, and (C) human spleen tissue with PD-1 antibody at 5 &956,g/mL. (D) Immunohistochemistry in human tonsil tissue with control mouse IgG staining at 5 &956,g/mL.

Immunohistochemistry of PD-1 in (A) human breast cancer tissue and (B) human normal breast tissue with PD-1 antibody at 5 &956,g/mL.

Western blot analysis of PD-1 in transfected 293 cell lysate with PD-1 antibody at 1 &956,g/mL.