PDL2 Detection Set (Risk Free), IgG1, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: PDL2 Detection Set (Risk Free), IgG1, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: PRS-RF16020
• Supplier Catalog Number: RF16020
• Alternative Catalog Number: PRS-RF16020-1
• Manufacturer: ProSci
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FC, ICC, IF, IHC-P, WB
• Species Reactivity: Human
• Immunogen: Mouse monoclonal PD-L2 antibodies were raised against the extracellular domain of human PD-L2.
• Conjugation: Unconjugated

Product Properties

• Clonality: Monoclonal
• Concentration: Antibody 1 mg/mL
• Isotype: IgG1
• Buffer: PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.

Images

Titration curve analysis of PD-L2 mAbs to detect recombinant PD-L2 in ELISA with RF16021, RF16022, RF16023, RF16024, and RF16025 abs at decreasing concentrations.

Flow cytometry analysis of PD-L2 overexpressing HEK293 cells using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 10 &956,g/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L2 overexpressing HEK293 cells.

Immunocytochemistry of PD-L2 in overexpressing 293 cells using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 5 &956,g/ml.

Immunofluorescence of PD-L2 in overexpressing 293 cells using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 20 &956,g/ml.

Immunofluorescence of PD-L2 in human tonsil tissue using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 20 &956,g/ml.

Immunohistochemistry of PD-L2 in human colon carcinoma tissue using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 2 &956,g/ml.

Immunohistochemistry of PD-L2 in human tonsil tissue using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 2 &956,g/ml.

Western blot analysis of PD-L2 in overexpressing 293 cells using RF16021, RF16022, RF16023, RF16024, and RF16025 antibody at 0.5 and 1 &956,g/ml, respectively. Larger molecular weight bands represent more highly post-translationally modified PD-L2.