PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Form:
Liquid
Application Dilute:
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
PD-L1 antibody can be used for detection of PD-L1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2 - 5 µg/mL. For immunofluorescence start at 5 µg/mL.Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Immunocytochemistry of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 1 &956,g/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 &956,g/mL.
Immunofluorescence of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 2 &956,g/mL. Red: PDL1 Antibody [2D6] (RF16036) Blue: DAPI staining
Immunofluorescence of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 2 &956,g/mL. Red: PDL1 Antibody [2D6] (RF16036) Blue: DAPI staining
Immunofluorescence of PD-L1 in human tonsil tissue with PD-L1 antibody at 2 &956,g/mL. Red: PDL1 Antibody [2D6] (RF16036) Blue: DAPI staining
Immunohistochemistry of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 5 &956,g/mL.
Immunohistochemistry of PD-L1 in human tonsil tissue with PD-L1 antibody at 5 &956,g/mL.
Western blot analysis of PD-L1 in overexpressing HEK293 cells PD-L1 antibody at 0.25 and 0.5 &956,g/ml
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