CD80 Antibody [12D9], IgG1, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: CD80 Antibody [12D9], IgG1, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: PRS-RF16046
• Supplier Catalog Number: RF16046
• Alternative Catalog Number: PRS-RF16046-0.02,PRS-RF16046-0.1
• Manufacturer: ProSci
• Host: Mouse
• Category: Antikörper
• Application: ELISA, ICC, IF, IHC-P, WB
• Species Reactivity: Human
• Immunogen: CD80 antibody was raised against the extracellular domain of human CD80.
• Conjugation: Unconjugated
• Alternative Names: CD80 Antibody: CD80 molecule, B7, BB1, B7-1, B7.1, LAB7, CD28LG, CD28LG1

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/mL
• Clone Designation: [12D9]
• Molecular Weight: Predicted: 32 kDa Observed: 50 kDa
• Isotype: IgG1
• NCBI: 941
• UniProt: P33681
• Buffer: CD80 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: CD80 antibody can be used for detection of CD80 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2 - 5 µg/mL. For immunofluorescence start at 5µg/mL.Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples, Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.

Images

Flow cytometry analysis of CD80 overexpressing HEK293 cells using CD80 antibody and control mouse IgG antibody at 10 &956,g/ml. Blue: Untransfected HEK293 cells. Yellow: CD80 overexpressing HEK293 cells.

Immunocytochemistry of CD80 in transfected HEK293 cells with CD80 antibody at 1 &956,g/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 &956,g/mL.

Immunofluorescence of CD80 in transfected HEK293 cells with CD80 antibody at 2 &956,g/mL. Red: CD80 Antibody [12D9] (RF16046) Blue: DAPI staining

Immunofluorescence of CD80 in human stomach carcinoma tissue with CD80 antibody at 20 &956,g/mL. Red: CD80 Antibody [12D9] (RF16046) Blue: DAPI staining

Immunofluorescence of CD80 in human tonsil tissue with CD80 antibody at 2 &956,g/mL. Red: CD80 Antibody [12D9] (RF16046) Blue: DAPI staining

Immunohistochemistry of CD80 in human stomach carcinoma tissue with CD80 antibody at 5 &956,g/mL.

Immunohistochemistry of CD80 in human tonsil tissue with CD80 antibody at 5 &956,g/mL.

Western blot analysis of CD80 in overexpressing HEK293 cells CD80 antibody at 0.25 and 0.5 &956,g/ml