LIGHT Antibody [7B9E12], IgG2b, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: LIGHT Antibody [7B9E12], IgG2b, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: PRS-RF16063
• Supplier Catalog Number: RF16063
• Alternative Catalog Number: PRS-RF16063-0.02,PRS-RF16063-0.1
• Manufacturer: ProSci
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FC, ICC, IF, IHC-P, WB
• Species Reactivity: Human
• Immunogen: LIGHT antibody was raised against the extracellular domain of human LIGHT.
• Conjugation: Unconjugated
• Alternative Names: LIGHT Antibody: TNFSF14, LTg, CD258, HVEML, HVEM ligand

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/mL
• Clone Designation: [7B9E12]
• Molecular Weight: Predicted: 26 kDa
• Isotype: IgG2b
• NCBI: 8740
• UniProt: O43557
• Buffer: LIGHT Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: LIGHT antibody can be used for detection of CD80 by Western blot at 0.5 - 1 µg/mL. LIGHT antibody can be used for immunohistochemistry starting at 2 - 5 µg/mL. For immunofluorescence start at 20 µg/mL.Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples, Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.

Images

Flow cytometry analysis of LIGHT overexpressing HEK293 cells using LIGHT antibody and control mouse IgG antibody at 10 &956,g/ml. Blue: Untransfected HEK293 cells. Yellow: LIGHT overexpressing HEK293 cells.

Immunocytochemistry of LIGHT in transfected HEK293 cells with LIGHT antibody at 1 &956,g/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 &956,g/mL.

Immunofluorescence of LIGHT in transfected HEK293 cells with LIGHT antibody at 2 &956,g/mL. Green: LIGHT Antibody [7B9E12] (RF16063) Blue: DAPI staining

Immunofluorescence of LIGHT in human lymphoma tissue with LIGHT antibody at 20 &956,g/mL. Green: LIGHT Antibody [7B9E12] (RF16063) Blue: DAPI staining

Immunohistochemistry of LIGHT in human lymphoma tissue with LIGHT antibody at 5 &956,g/mL.

Western blot analysis of LIGHT in overexpressing HEK293 cells with LIGHT antibody at 0.5 and 1 &956,g/ml