VISTA Detection Set (Risk Free), Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: VISTA Detection Set (Risk Free), Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: PRS-RF16070
• Supplier Catalog Number: RF16070
• Alternative Catalog Number: PRS-RF16070-1
• Manufacturer: ProSci
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FC, ICC, IF, IHC-P, WB
• Species Reactivity: Human
• Immunogen: VISTA antibodies were raised against the extracellular domain of human VISTA.
• Conjugation: Unconjugated

Product Properties

• Clonality: Monoclonal
• Concentration: Antibody 1 mg/mL
• Buffer: PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.

Images

Titration curve analysis of VISTA mAbs to detect recombinant LVISTA in ELISA with RF16071, RF16072, RF16073, and RF16074 abs at decreasing concentrations.

Flow cytometry analysis of VISTA over expressing HEK293 cells using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 10 &956,g/ml. Blue: untransfected HEK293 cells. Yellow: VISTA over expressing HEK293 cells.

Immunocytochemistry of VISTA in over expressing HEK293 cells using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 1 &956,g/ml.

Immunofluorescence of VISTA in over expressing HEK293 cells using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 2 &956,g/ml.

Immunofluorescence of VISTA in human spleen tissue using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 10 &956,g/ml.

Immunohistochemistry of VISTA in human lymphoma tissue using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 2 &956,g/ml.

Western blot analysis of VISTA in over expressing HEK293 cells using RF16071 antibody at (A) 0.25 µg/ml , (B) 0.5 µg/ml, and (C) 1 &956,g/ml.