LAG3 Detection Set (Risk Free), Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: LAG3 Detection Set (Risk Free), Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: PRS-RF16080
• Supplier Catalog Number: RF16080
• Alternative Catalog Number: PRS-RF16080-1
• Manufacturer: ProSci
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FC, ICC, IF, IHC-P, WB
• Species Reactivity: Human
• Immunogen: LAG3 antibodies were raised against the extracellular domain of human LAG3.
• Conjugation: Unconjugated

Product Properties

• Clonality: Monoclonal
• Concentration: Antibody 1 mg/mL
• Buffer: PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.

Images

A sandwich ELISA was performed using the anti-LAG3 mAbs RF16082, RF16083, RF16084, RF16086, RF16087 and RF16088 as the capture antibodies for the LAG3 extracellular domain antigen with biotin-labeled Risk-Free anti-LAG3 mAbs as the detection antibodies.

Flow cytometry analysis of LAG-3 over expressing HEK293 cells using (A) RF16082, (B) RF16083, (C) RF16084, (D) RF16086, (E) RF16087, (F) RF16088, (G) RF16089, and (H) control mouse IgG antibody at 1 &956,g/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.

Immunocytochemistry of LAG-3 in over expressing HEK293 cells using (A) RF16082, (B) RF16083, (C) RF16084, (D) RF16086, (E) RF16087, (F) RF16088, (G) RF16089, and (H) control mouse IgG antibody at 1 &956,g/ml.

Immunofluorescence of LAG-3 in over expressing HEK293 cells using (A) RF16082, (B) RF16083, (C) RF16084, (D) RF16086, (E) RF16087, (F) RF16088, (G) RF16089, and (H) control mouse IgG antibody at 2 &956,g/ml.

Immunofluorescence of LAG-3 in human spleen tissue using (A) RF16082, (B) RF16084, (C) RF16086, (D) RF16087, (E) RF16088, (F) RF16089, and (G) control mouse IgG antibody at 10 &956,g/ml.

Immunohistochemistry of LAG-3 in human lymphoma tissue using (A) RF16082, (B) RF16084, (C) RF16086, (D) RF16087, (E) RF16088, (F) RF16089, and (G) control mouse IgG antibody at 5 &956,g/ml.

Western blot analysis of LAG-3 in over expressing HEK293 cells using RF16082, RF16088, and RF16089 antibodies at (A) 0.25 (B) 0.5 and (C) 1 &956,g/ml.

Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA with RF16082, RF16083, RF16084, RF16086, RF16087, RF16088, and RF16089 antibodies at decreasing concentrations.