SARS-CoV-2 S protein RBD containing C-terminal His Tag. The protein was expressed in human 293 cells (HEK293). It contains amino acids Arg 319 - Lys 537.
SARS-CoV-2 (COVID-19) S1 RBD Antibody [T5P8-F9] Antibody is supplied in PBS.
Form:
Liquid
Application Dilute:
Optimal dilutions for each application to be determined by the researcher.
Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) Spike Trimer Proteins of Omicron and Delta Variants Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibodies. A direct ELISA was performed using SARS-CoV-2 Spike Trimer recombinant proteins of Omicron and Delta variants as coating antigen at 1 &956,g/mL and each of the 6 anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibodies as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1 &956,g/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SD9439, SD9441, and SD9503 bind spike proteins of omicron variant, but not delta variant, SD9511 and SD9437 bind spike proteins of delta variant, but not omicron variant, SD9507 binds spike proteins of delta and omicron variants.
Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) Spike RBD and S1 Proteins Antibodies: SARS-CoV-2 (COVID-19) Spike RBD Antibody, SD9503. A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, 10-303, S1, 97-087) as coating antigen at 1 &956,g/mL and the anti-SARS-CoV-2 (COVID-19) Spike RBD antibody (SD9503) as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1 &956,g/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 (COVID-19) Spike RBD Antibody, SD9503 can detect spike RBD protein at 10 ng/mL and can weakly detect spike S1 protein at 200 ng/mL.
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