SARS-CoV-2 Spike S2 protein containing C-terminal His Tag. The protein was expressed in human 293 cells (HEK293). It contains amino acids Ser 686 - Pro 1213.
SARS-CoV-2 (COVID-19) Spike S2 Antibody is supplied in PBS.
Form:
Liquid
Application Dilute:
Optimal dilutions for each application to be determined by the researcher.
Figure 1 ELISA Validation with Spike Trimer Proteins of SARS-CoV-2 Variants and SARS-CoV-1 Antibodies: SARS-CoV-2 (COVID-19) Spike S2 Antibody, SD9785. A direct ELISA was performed using Spike trimer protein of SARS-CoV-2 Variants (Delta and Omicron) and SARS-CoV-1 as coating antigens at 1 &956,g/mL and the anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (SD9785) as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1 &956,g/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 16 ng/mL to 2000 ng/mL.
Figure 2 Epitope Mapping of four Spike S2 Antibodies by Direct Competitive ELISA A direct competitive ELISA was developed for the epitope mapping using Spike trimer proteins of SARS-CoV-2 Delta variant at 1 &956,g/ml as the coating antigen and biotinylated Spike S2 single domain antibodies (sdAbs) as the competitive antibodies. The sdAb mixtures (biotinylated and unconjugated sdAbs) were added to ELISA plate and streptavidin-HRP was used to detect the captured biotinylated sdAbs. The assay suggests the antibodies bind to different epitopes of the Spike trimer.
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