Optimal dilutions for each application to be determined by the researcher.
Application Notes:
Clusterin antibody can be used for detection of human clusterin by immunocytochemistry, immunohistochemistry (frozen sections), immunoprecipitation, and western blot (1:2,000). Method: : Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2,000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.
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