DR6 Antibody [Luke-1], IgG2a, Unconjugated, Rat, Monoclonal
Catalog Number:
PRS-XA-1015
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| Article Name: | DR6 Antibody [Luke-1], IgG2a, Unconjugated, Rat, Monoclonal |
| Biozol Catalog Number: | PRS-XA-1015 |
| Supplier Catalog Number: | XA-1015 |
| Alternative Catalog Number: | PRS-XA-1015-0.1 |
| Manufacturer: | ProSci |
| Host: | Rat |
| Category: | Antikörper |
| Application: | FC |
| Species Reactivity: | Human |
| Immunogen: | DR6 (monoclonal Luke-1) antibody was raised against recombinant human DR6,Fc (aa. 42-335). |
| Conjugation: | Unconjugated |
| Application Dilute: | Optimal dilutions for each application to be determined by the researcher. |
| Application Notes: | DR6 antibody can be used to recognize human DR6 by Flow Cytometry. Method: Cells (5x105) were incubated on ice for 30 min in 50 µl FACS buffer (PBS, 5% FCS, 0.02% azide) containing 1 µg/mL of anti-DR6 MAb (Luke-1). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry. Method: 3 HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mDR6 (thick line). Cells were incubated on ice for 30 min in 50 µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 µg/mL of Luke-1 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry. |
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