RIP3 Antibody [Rippy-3], IgG1, Unconjugated, Mouse, Monoclonal

Catalog Number: PRS-XA-1023
Article Name: RIP3 Antibody [Rippy-3], IgG1, Unconjugated, Mouse, Monoclonal
Biozol Catalog Number: PRS-XA-1023
Supplier Catalog Number: XA-1023
Alternative Catalog Number: PRS-XA-1023-0.1
Manufacturer: ProSci
Host: Mouse
Category: Antikörper
Application: WB
Species Reactivity: Human
Immunogen: RIP3 (monoclonal Rippy-3) antibody was raised against recombinant human RIP3.
Conjugation: Unconjugated
Clonality: Monoclonal
Concentration: batch dependent
Clone Designation: [Rippy-3]
Isotype: IgG1
NCBI: 11035
UniProt: Q9Y572
Buffer: PBS containing 0.02% sodium azide
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: RIP3 antibody can be used to recognize human RIP3 by western blot. Method: HEK 293T cells (5x105) mock transfected (lane 1) or transfected (lane 2) with an expression plasmid coding for human RIP3 were harvested, lysed and cell extracts (5x105) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the Rippy-3 monoclonal antibody at a 1:1000 dilution for 2 hours. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG and a chemiluminescence detection system. This product is for research use only.