Optimal dilutions for each application to be determined by the researcher.
Application Notes:
RIP2 antibody can be used to recognize human RIP2 by Western Blot. Method: Cell extracts (5x105) from HEK 293T wild type (lane 1) or transfected with a plasmid coding for human RIP2 (lane 2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the MAb to RIP2 (Nick-1) at a 1:1000 dilution for 2 hours. Proteins were visualized using a peroxidase-conjugated polyclonal antibody to rat IgG and a chemiluminescence detection system. This product is for research use only.
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