IL-6 Antibody (biotin), Biotin, Rabbit, Polyclonal

Product Overview

• Article Name: IL-6 Antibody (biotin), Biotin, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-XP-5195BT
• Supplier Catalog Number: XP-5195Bt
• Alternative Catalog Number: PRS-XP-5195BT-0.05
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Rat
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat IL-6 (rat IL-6).
• Conjugation: Biotin
• Alternative Names: ILg6, Ifnb2, Il-6, Interleukin-6IL-6

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• NCBI: 24498
• UniProt: P20607
• Form: Lyophilized

Application Notes

• Application Dilute: Centrifuge vial prior to opening.
• Application Notes: ELISA:Sandwich:To detect Rat IL-6 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25 - 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat IL-6 (XP-5195) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-6. Western Blot:To detect rat IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.