ELISA:To detect mLIX by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mLIX. Sandwich:To detect mLIX by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine LIX (XP-5216Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLIX.Western Blot:To detect mLIX by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLIX is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
* VAT and and shipping costs not included. Errors and price changes excepted