LIX Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-XP-5216
Article Name: LIX Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-XP-5216
Supplier Catalog Number: XP-5216
Alternative Catalog Number: PRS-XP-5216-0.1
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Mouse
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mLIX (murine LIX).
Conjugation: Unconjugated
Alternative Names: LIX, GCP-2, Scyb5, Scyb6, ENA-78, AMCF-II, C-X-C motif chemokine 5, Cytokine LIX,
Clonality: Polyclonal
Concentration: batch dependent
NCBI: 20311
UniProt: P50228
Form: Lyophilized
Application Dilute: Centrifuge vial prior to opening.
Application Notes: ELISA:To detect mLIX by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mLIX. Sandwich:To detect mLIX by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine LIX (XP-5216Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLIX.Western Blot:To detect mLIX by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLIX is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.