MCP-4 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: MCP-4 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-XP-5224
• Supplier Catalog Number: XP-5224
• Alternative Catalog Number: PRS-XP-5224-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, NeA, WB
• Species Reactivity: Human
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMCP-4 (human Macrophage/Monocyte chemotactic protein-4).
• Conjugation: Unconjugated
• Alternative Names: NCC1, CKb10, MCP-4, NCC-1, SCYL1, SCYA13, MCP4, NCC1, C-C motif chemokine 13, CK-beta-10

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• NCBI: 6357
• UniProt: Q99616
• Form: Lyophilized

Application Notes

• Application Dilute: Centrifuge vial prior to opening.
• Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-4 (30.00 ng/mL), a concentration of 3.0 - 4.0 µg/mL of this antibody is required. ELISA:To detect hMCP-4 by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMCP-4.Sandwich:To detect hMCP-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MCP-4 (XP-5224Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-4. Western Blot:To detect hMCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.