TGF-a Antibody (biotin), Biotin, Rabbit, Polyclonal

Catalog Number: PRS-XP-5281BT
Article Name: TGF-a Antibody (biotin), Biotin, Rabbit, Polyclonal
Biozol Catalog Number: PRS-XP-5281BT
Supplier Catalog Number: XP-5281Bt
Alternative Catalog Number: PRS-XP-5281BT-0.05
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTGF-alpha (human hTGF-alpha).
Conjugation: Biotin
Alternative Names: TFGAProtransforming growth factor alpha, EGF-like TGF, TGF-alpha
Clonality: Polyclonal
Concentration: batch dependent
NCBI: 7039
UniProt: P01135
Form: Lyophilized
Application Dilute: Centrifuge vial prior to opening.
Application Notes: ELISA:Sandwich:To detect hTGF-alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25 - 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human TGF-alpha (XP-5281) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTGF-alpha.Western Blot:To detect hTGF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTGF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.