Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL 7 (0.5 ng/mL), a concentration of 0.05-0.08 µg/mL of this antibody is required. ELISA:In a sandwich ELISA (assuming 100 µL/well), a concentration of 2.0-4.0 µg/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-7 when used with our biotinylated antigen affinity purified anti-human IL-7 (XP-5197Bt) as the detection antibody at a concentration of approximately 0.5-1.0 µg/mL.Western Blot:To detect hIL-7 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-7 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
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