ELISA:In a sandwich ELISA (assuming 100 µL/well), a concentration of 2.0-4.0 µg/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-8 when used with our biotinylated antigen affinity purified anti-human IL-8 (XP-5199Bt) as the detection antibody at a concentration of approximately 0.5-1.0 µg/mL.Western Blot: To detect hIL-8 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-8 is 2.0-4.0 ng/lane, under non-reducing conditions.
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