ELISA:In a sandwich ELISA (assuming 100 µL/well), a concentration of 2.0-4.0 µg/mL of this antibody will detect at least 500 pg/mL of recombinant human NAP-2 when used with our biotinylated antigen affinity purified anti-human NAP-2 (XP-5244Bt) as the detection antibody at a concentration of approximately 0.5-1.0 µg/mL. Western blot:To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
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