ELISA:In a sandwich ELISA (assuming 100 µL/well), a concentration of 2.0-4.0 µg/mL of this antibody will detect at least 500 pg/mL of recombinant human LEC when used with our biotinylated antigen affinity purified anti-human LEC (XP-5722-M) as the detection antibody at a concentration of approximately 0.5-1.0 µg/mL. Western Blot:To detect hLEC by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLEC is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
* VAT and and shipping costs not included. Errors and price changes excepted