TOP1 70kDa is supplied in 16mM HEPES buffer pH-8.0, 400mM NaCl, and 20% glycerol.
Application Dilute:
Western blot with Scleroderma patient sera or monoclonal anti-hexa-His-tag antibody. Standard ELISA test (checkerboard analysis of positive/negative sera panels, including CDC international reference sera). (0.5-0.8 ug/ml depending on the type of ELISA pl
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