Recombinant human Interleukin-21 receptor/IL-21R overexpression cell lysate, derived from the transfected HEK293 cells. (100 µg). Purchase will also include one vial of normal control HEK293 cell lysate (Catalog 230-10006) transfected with empty expression vector.
Concentration:
Determined by BCA protein assay kit (Thermo Scientific)
Molecular Weight:
Recombinant protein has a calculated molecular mass of 25. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs).
Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control.
Form:
Lyophilized powder
Sequence:
Cys20-Glu232
Formula:
Lyophilized from a 0.2 µm filtered solution in PBS containing mammalian cell protease inhibitor cocktail
Application Notes:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
* VAT and and shipping costs not included. Errors and price changes excepted
