Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, Hypoxia Inducible Factor 1, Alpha Subunit, Basic Helix-Loop-Helix Transcription Factor, HIF-1alpha, MOP1, HIF1, ARNT Interacting Protein, Hypoxia-Inducible Factor 1 Alpha Isoform I.3, PASD8, Hypoxia-Inducible Factor1alpha, Member Of PAS Superfamily 1, PAS Domain-Containing Protein 8, BHLHE78, HIF-1A
Biotin-conjugated recombinant Human HIF-1-alpha, derived from the transfected human HEK293 cells
Concentration:
Determined by BCA protein assay kit (Thermo Scientific)
Molecular Weight:
The recombinant human HIF-1-alpha, C-terminal domain, has a calculated molecular mass of 49 kDa. Due to the abundant post-translation modifications, it migrates as approximately 60-80 kDa protein bands (confirmed by Western blotting) in SDS-PAGE under DTT
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Form:
Lyophilized powder
Sequence:
Leu400-Asn826
Formula:
Lyophilized from a 0.2 µm filtered solution in PBS
Application Notes:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
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