Recombinant Human CEA, derived from the transfected human HEK293 cells
Concentration:
Determined by BCA protein assay kit (Thermo Scientific)
Molecular Weight:
The recombinant human CEA has a calculated molecular mass of 71 kDa. Due to the abundant glycosylation, it migrates as approximately 125-150 kDa protein bands (confirmed by Western blotting) in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Tag:
His
Expression System:
HEK293 cells
Purity:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Form:
Lyophilized powder
Sequence:
Lys35-Ala685
Formula:
Lyophilized from a 0.2 µm filtered solution in PBS
Application Notes:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
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