Osteopontin, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin, Early T-Lymphocyte Activation 1, BNSP, OPN, Secreted Phosphoprotein 1 (Osteopontin, Bone Sialoprotein I, Early T-Lymphocyte Activation 1), Osteopontin/Immunoglobulin Alpha 1 Heavy Chain Constant Region Fusion Protein 3, Secreted Phosphoprotein 1 Variant 6, SPP1/CALPHA1 Fusion, Bone Sialoprotein 1, ETA-1, BSPI
Recombinant Human Osteopontin, derived from the transfected human HEK293 cells.
Concentration:
Determined by Pierce BCA protein assay kit
Molecular Weight:
Recombinant Human Osteopontin has a calculated molecular mass of 32 kDa. Due to the abundant glycosylation, it migrates as approximately 50-55 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Tag:
His
Expression System:
HEK293 cells
Purity:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Form:
Lyophilized powder
Sequence:
Ile17-Asn300
Formula:
Lyophilized from a 0.2 µm filtered solution in PBS
Application Notes:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
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