Biotin-conjugated recombinant human ROBO1 protein, N-terminal region with a C-terminal His-tag, derived from the transfected human HEK293 cells.
Concentration:
Determined by BCA protein assay
Molecular Weight:
Recombinant protein product has a calculated molecular mass of 95kDa. Due to the abundant glycosylation, it migrates as approximately 110 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native
Tag:
His
Expression System:
HEK293 cells
Purity:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Form:
Lyophilized powder
Sequence:
Ser20-Pro861
Formula:
Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4)
Application Notes:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
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