Biotin-conjugated recombinant human neuropilin-1 (NRP1, isoform 1), triple mutant (S346A, E348A, T349A) with C-terminal His-tag, derived from the transfected human HEK293 cells.
Concentration:
Lot specific (see the label on the vial), determined by BCA protein assay
Molecular Weight:
Recombinant protein product has a calculated molecular mass of 95 kDa. Due to the abundant glycosylation, it migrates as approximately 120 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under nativ
Tag:
His
Expression System:
HEK293 cells
Purity:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Form:
Liquid
Sequence:
Phe22-Pro856. Three amino acid residue at Ser346, Glu348 and Thr349 were mutated to Ala (A) and generated NRP1 triple mutant (S346A, E348A, and T349A).
Formula:
Supplied as a 0.2 µm filtered solution in PBS (pH 7.4)
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