Biotin-conjugated recombinant SARS-CoV-2 spike S1 subunit protein, Y145H/A222V mutant, with C-terminal His-tag, derived from the transfected human HEK293 cells.
Concentration:
Lot specific (see the label on the vial), determined by BCA protein assay
Molecular Weight:
Recombinant protein product has a calculated molecular mass of 75 kDa. Due to the abundant glycosylation, it migrates as approximately 120 kDa major protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Tag:
His
Expression System:
HEK293 cells
Purity:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Form:
Liquid
Sequence:
Val16-Gln690. Y145H/A222V mutant: amino acid Tyr (Y) at 145 position was mutated to His (H), Ala (A) at 222 position was mutated to Val (V).
Formula:
Supplied as a 0.2 µm filtered solution in PBS (pH 7.4)
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