Recombinant SARS-CoV-2 S1 subunit protein RBD, mutant R346K/E484K/N501Y with C-terminal His-tag, derived from the transfected human HEK293 cells.
Concentration:
Lot specific (see the label on the vial), determined by BCA protein assay
Molecular Weight:
Recombinant protein product has a calculated molecular mass of 25 kDa. Due to the abundant glycosylation, it migrates as approximately 30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native
Tag:
His
Expression System:
HEK293 cells
Purity:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Form:
Liquid
Sequence:
Arg319-Phe541. R346K/E484K/N501Y mutant: amino acid Arg (R) at 346 position was mutated to Lys (K), Glu (E) at 484 position was mutated to Lys (K), Asn (N) at 501 position was mutated to Tyr (Y).
Formula:
Supplied as a 0.2 µm filtered solution in PBS (pH 7.4)
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