Immunogen was full recombinant protein derived from human TNFalpha. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography.
Mouse anti-TNF alpha Biotinylated Antibody, 50 ug
Clonality:
Monoclonal
Isotype:
IgG1
Tag:
Biotin
UniProt:
NM_000595
Application Notes:
Direct ELISA: antibody detection limit dilution 1:10,000-20,000, Recommended for use at 5-10 µg/ml., Western Blot: Approx 26 and 17 kDa band observed in Jurkat cell lysate. this antibody can be used at 1 - 2 µg/ml with the appropriate secondary reagents to detect human TNF-alpha.,
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