The immunogen was a synthetic peptide corresponding to the N-terminal region of Bcl-2. This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified from rabbit serum by Protein G affinity chromatography.
Rabbit Anti-Human bcl-2 Antibody
Clonality:
Polyclonal
UniProt:
NP_000624
Sequence:
CMKYIHYKLSQRGYEWDAGD
Application Notes:
ELISA (recommended work dilution= 1:40000 (detects ?25ng/ml) ), Western Blotting (recommended work dilution= 1: 5,000), (see image)Western blot analysis of 100 ng Bcl-2 using purified rabbit anti-Bcl-2 primary antibody (1:5,000 µg/ml). The blot was incubated with anti-rabbit HRP-conjugated IgG (1:10,000).
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