Immunogen was synthetic peptide derived from Human Vaspin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from goat serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography.
Rabbit Anti-Human Vaspin Antibody
Clonality:
Polyclonal
UniProt:
NM_173850
Application Notes:
Direct and indirect ELISA: antibody detection limit dilution at least 1:1,000-2,000. Western Blot: Reacted with the approx 48-kDa Vaspin His tagged protein. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature. Recommended for use at dilution 1:500-1,000.
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