(KO Validated) p53 Polyclonal Antibody, Unconjugated, Rabbit

Product Overview

• Article Name: (KO Validated) p53 Polyclonal Antibody, Unconjugated, Rabbit
• Biozol Catalog Number: RDD-RD90322A
• Supplier Catalog Number: RD90322A
• Alternative Catalog Number: RDD-RD90322A-60,RDD-RD90322A-120,RDD-RD90322A-200
• Manufacturer: reddot Biotech
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human
• Immunogen: Recombinant fusion protein of human p53
• Conjugation: Unconjugated
• Alternative Names: TP53, BCC7, LFS1, P53, TRP53, tumor protein p53

Product Description

This is a (KO Validated) p53 Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/mL
• Molecular Weight: Observed MW: 43KDa. Observed MW: 43KDa
• Buffer: PBS with 0.01% thiomersal,50% glycerol,pH7.3.

Images

Confocal immunofluorescence analysis of Hela cells using p53 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of U2OS cells using p53 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.

Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] p53 Polyclonal Antibody at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug p53 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using p53 Polyclonal Antibody at a dilution of 1:3000.

Western blot analysis of extracts of 293T cells using p53 Polyclonal Antibody at 1:1000 dilution.

Western blot analysis of extracts from wild type(WT) and p53 knockout (KO) 293T cells using p53 Polyclonal Antibodyat 1:1000 dilution.

Antibodies from Reddot Biotech