All Lanes: HDAC4 Antibody at 1/ 500 dilution.Lane1:Hela lysate.Proteins at 20µg per laneSecondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 140kDaObserved band size: 140kDa
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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