Triple FLAG Peptide

Product Overview

• Article Name: Triple FLAG Peptide
• Biozol Catalog Number: ROC-000-000-H47
• Supplier Catalog Number: 000-000-H47
• Alternative Catalog Number: ROC-000-000-H47
• Manufacturer: Rockland Immunochemicals
• Category: Proteine/Peptide
• Application: SDS-PAGE
• Conjugation: Unconjugated
• Alternative Names: synthetic FLAG triple peptide, Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide, control peptide, blocking peptide

Product Properties

• Concentration: 1.0 mg/ml by dry weight
• Buffer: None
• Form: Lyophilized

Application Notes

• Application Dilute: IP: YES - see PROTOCOL, WB: YES as blocking peptide in PCA
• Application Notes: The Triple FLAG Peptide has been tested by SDS-PAGE. A working concentration of 500 µg/mL for triple FLAG peptide is typically used for competitive elution of FLAG containing proteins from anti-DYKDDDDK affinity gels (p/n 200-350-383). For use as a co

Images

SDS-PAGE of Anti-DYKDDDDK (FLAG tag) Affinity Gel. Lane 1: DYKDDDDK tagged recombinant protein. Lane 2: flow through. Lane 3: wash. Lane 4: eluted fraction one. Lane 5: eluted fraction two. Lane 6: eluted fraction three. Lane 7: blank. Lane 8: molecular weight markers. Load: 5 µg protein lane 1, 15µL per each other lane. Predicted/Observed size: 135kDa for DYKDDDDK tagged recombinant protein.