Bovine Collagen Type VI

Product Overview

• Article Name: Bovine Collagen Type VI
• Biozol Catalog Number: ROC-001-001-108
• Supplier Catalog Number: 001-001-108
• Alternative Catalog Number: ROC-001-001-108
• Manufacturer: Rockland Immunochemicals
• Category: Sonstiges
• Application: DOT
• Conjugation: Unconjugated
• Alternative Names: Collagen alpha 1 (VI) chain, COL6A1, Bovine Collagen Type VI

Product Properties

• Concentration: 1.0 mg/ml by modified Lowry assay
• Buffer: 0.5 M Acetic Acid
• Source: Bovine
• Form: Liquid (sterile filtered)

Application Notes

• Application Dilute: ELISA: 1:10,000, IHC: 1:100-1:500, WB: 1:1000
• Application Notes: For use as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.

Images

Dot Blot results using Rabbit Anti-Collagen VI Antibody. Sample: A) Human Collagen VI (p/n 009-001-108), B) Bovine/Human Collagen VI (p/n 001-001-108).Load: 1) 1000ng, 2) 333.33ng, 3) 111.11ng, 4) 37.03ng, 5) 12.35ng.Primary Antibody: Rabbit Anti-Collagen VI antibody (p/n 600-401-108) at 5µg/mL at RT for 1hr. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated (p/n 611-1302) 1:40,000 at RT for 30mins. Blocking: BlockOut Universal Blocking Buffer (p/n MB-073) at RT for 30mins.