Chicken IgG Fab

Product Overview

• Article Name: Chicken IgG Fab
• Biozol Catalog Number: ROC-003-0105-0002
• Supplier Catalog Number: 003-0105-0002
• Alternative Catalog Number: ROC-003-0105-0002
• Manufacturer: Rockland Immunochemicals
• Category: Antikörper
• Application: SDS-PAGE
• Conjugation: Unconjugated
• Alternative Names: Chicken IgG Fab fragment, Chicken IgY Fab fragment

Product Properties

• Concentration: 2.0 mg/ml by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Source: Chicken
• Form: Liquid (sterile filtered)

Application Notes

• Application Notes: Chicken IgG F(ab) has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Images

SDS-PAGE of Chicken IgG/IgY Whole Molecule Rhodamine Conjugated (p/n 003-0002). Lane M: 5 µL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Chicken IgG Whole Molecule Rhodamine Conjugated (p/n 003-0002). Lane 2: Reduced Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Reduced Chicken IgG Fab Fragment (p/n 003-0105). Lane 4: Reduced Chicken IgM Whole Molecule (p/n 003-0107). Load: 1 µg per lane. Predicted/Observed size: IgG at 72 and 25 kDa, F(c) at 25 kDa, Fab at 25 kDa, IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.