Chicken IgM

Product Overview

• Article Name: Chicken IgM
• Biozol Catalog Number: ROC-003-0107
• Supplier Catalog Number: 003-0107
• Alternative Catalog Number: ROC-003-0107
• Manufacturer: Rockland Immunochemicals
• Category: Antikörper
• Application: SDS-PAGE
• Conjugation: Unconjugated
• Alternative Names: Chicken Immunoglobulin M, Fowl IgM

Product Properties

• Concentration: 1.0mg/ml by UV absorbance at 280 nm
• Isotype: IgM
• Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
• Source: Chicken
• Form: Liquid (sterile filtered)

Application Notes

• Application Dilute: ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
• Application Notes: Chicken IgM whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Images

SDS-PAGE of Chicken IgG/IgY Whole Molecule Rhodamine Conjugated (p/n 003-0002). Lane M: 5 µL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Chicken IgG Whole Molecule Rhodamine Conjugated (p/n 003-0002). Lane 2: Reduced Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Reduced Chicken IgG Fab Fragment (p/n 003-0105). Lane 4: Reduced Chicken IgM Whole Molecule (p/n 003-0107). Load: 1 µg per lane. Predicted/Observed size: IgG at 72 and 25 kDa, F(c) at 25 kDa, Fab at 25 kDa, IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.