Goat IgG Alkaline Phosphatase, ALP

Product Overview

• Article Name: Goat IgG Alkaline Phosphatase, ALP
• Biozol Catalog Number: ROC-005-0502
• Supplier Catalog Number: 005-0502
• Alternative Catalog Number: ROC-005-0502
• Manufacturer: Rockland Immunochemicals
• Category: Antikörper
• Application: DOT
• Conjugation: ALP
• Alternative Names: Goat IgG alkaline phosphatase conjugated, Goat IgG alk phos conjugated

Product Properties

• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Source: Goat
• Form: Liquid (sterile filtered)

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Goat IgG whole molecule alkaline phosphatase conjugate has been tested in Dot Blot and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Images

SDS-PAGE of Goat IgG Whole Molecule Rhodamine Conjugated (p/n 005-0002). Lane M: 5 µL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Goat IgG Whole Molecule Rhodamine Conjugated (p/n 005-0002). Lane 2: Reduced Goat IgG F(c) Fragment (p/n 005-0103). Lane 3: Reduced Goat IgG F(ab) Fragment (p/n 005-0105). Lane 4: Reduced Goat IgM Whole Molecule (p/n 005-0107). Load: 1 µg for IgG, F(c) and F(ab), 3 µg for IgM. Predicted/Observed size: IgG at 50 and 25 kDa, F(c) at 25 kDa, F(ab) at 25 kDa, IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.