0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Source:
Goat
Form:
Lyophilized
Application Notes:
Goat IgG F(ab) fragment biotin conjugate has been tested by SDS-PAGE and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
SDS-PAGE of Goat IgG Whole Molecule Rhodamine Conjugated (p/n 005-0002). Lane M: 5 µL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Goat IgG Whole Molecule Rhodamine Conjugated (p/n 005-0002). Lane 2: Reduced Goat IgG F(c) Fragment (p/n 005-0103). Lane 3: Reduced Goat IgG F(ab) Fragment (p/n 005-0105). Lane 4: Reduced Goat IgM Whole Molecule (p/n 005-0107). Load: 1 µg for IgG, F(c) and F(ab), 3 µg for IgM. Predicted/Observed size: IgG at 50 and 25 kDa, F(c) at 25 kDa, F(ab) at 25 kDa, IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
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