Transforming Growth Factor beta 1 Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Transforming Growth Factor beta 1 in immunological assays.
Bioactivity of Human Transforming Growth Factor beta 1 Recombinant Protein. Serial dilutions of Human TGF-Beta 1 (starting at 10 ng/mL) were added to HT-2 cultured with IL-4. Cell proliferation was measured and the linear portion of the curve was us used to calculate the ED50. The ED50 of Human TGF-Beta 1 is between 0.1-0.15 ng/mL. This is comparable to the expected range of 0.04-0.2 ng/mL for this assay.
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