Human IgG CY5 conjugated, Cy5 isotype control for flow cytometry
Concentration:
1.0 mg/mL by UV absorbance at 280 nm
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Application Dilute:
Flow Cytometry: User Optimized, IHC: User Optimized, WB: User Optimized
Application Notes:
Human IgG whole molecule CY5 conjugated has been tested in dot blot and can be utilized as a control reagent in Flow Cytometry, Immunohistochemistry, and Western Blotting. Researchers should determine optimal titers for applications that are not stated.
Dot Blot Results of Human IgG Whole Molecule Cy5 Conjugated. Membrane: 0.45µm LF-PVDF. Blotted: Human IgG Whole Molecule Cy5 Conjugated at 3-fold dilution: 100ng, 33.33ng, 11.11ng, 3.7ng, 1.23ng. Blocked: Universal Blocking Buffer (p/n MB-073) for 30 min at RT.
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