Human immunoglobulin M, Human IgM (myeloma) whole molecule, Human IgM whole molecule, Hu IgM WM
Concentration:
2.25 mg/mL by UV absorbance at 280 nm
Isotype:
IgM
Buffer:
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Source:
Human
Form:
Liquid (sterile filtered)
Application Dilute:
ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
Application Notes:
Human IgM whole molecule has been tested in SDS-Page and can be used as a control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting.
SDS-PAGE of Human IgM Whole Molecule. Lane 1: Human IgM, Reduced. M: Opal Pre-stained Marker (MB-210-0500). Lane 2: Human IgM, Non-Reduced. Load: 1.0 µg per lane. Predicted/Observed size - Non-Reduced: 900 kDa, Reduced - 75, 25 kDa.
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