Human Immunoglobulin G F(c) fragment, IgG Fc, Vitamin H, Coenzyme R
Concentration:
1.0 mg/mL by UV absorbance at 280 nm
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Source:
Human
Form:
Lyophilized
Application Dilute:
ELISA: 1:5000 - 1:50,000, IHC: 1:500, WB: 1:1000
Application Notes:
Human IgG F(c) fragment Biotin conjugated has been tested in dot blot and can be utilized as a control reagent in both Western Blotting and ELISA format experiments.
Dot Blot of Human IgG F(c) Fragment Biotin Conjugated. Human IgG F(c) Biotin Conjugate (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Streptavidin HRP (p/n S000-03) at 1:40,000 for 30 mins at RT. Block: BlockOut buffer (p/n MB-073) at RT for 30 mins. Exposure: 1 sec.
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