Interleukin-6 (IL-6) has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Interleukin-6 (IL-6) in immunological assays. It is recommended to reconstitute the lyophilized recombinant M-IL-6 in 10 mM HCl to 0.1-1.0 mg/
SDS-PAGE using Rocklands Recombinant Mouse IL-6 Protein shows bands corresponding to IL-6 (1µg) in lane 1 (unreduced, arrowhead) and lane 3 (reduced). Molecular weight estimation was made by comparison to prestained MW markers, lane 2.
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