0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Source:
Mouse
Form:
Lyophilized
Application Dilute:
ELISA: User Optimized, FLISA: User Optimized, Flow Cytometry: 1:1000-1:5000, IF Microscopy: User Optimized, WB: User Optimized
Application Notes:
Mouse IgG3 isotype has been tested in dot blot and is designed for immunofluorescence microscopy, flow cytometry, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various c
Dot Blot showing the detection Fluorescein conjugated Mouse IgG3 subclass. A three-fold serial dilution of Fluorescein conjugated Mouse IgG3 subclass starting at 200ng was spotted onto 0.45 ?m nitrocellulose and imaged using the Bio-Rad VersaDoc 4000 MP.
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