Mouse IgG Fc Biotin
Catalog Number:
ROC-010-0603
| Article Name: |
Mouse IgG Fc Biotin |
| Biozol Catalog Number: |
ROC-010-0603 |
| Supplier Catalog Number: |
010-0603 |
| Alternative Catalog Number: |
ROC-010-0603 |
| Manufacturer: |
Rockland Immunochemicals |
| Category: |
Antikörper |
| Application: |
DOT |
| Conjugation: |
Biotin |
| Alternative Names: |
Mouse IgG F(c) Fragment Biotin Conjugation, Mouse IgG Fc Fragment Biotin Conjugated |
| Concentration: |
1.0 mg/mL by UV absorbance at 280 nm |
| Buffer: |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Source: |
Mouse |
| Form: |
Lyophilized |
| Application Notes: |
Mouse IgG F(c) fragment has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. |
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Dot Blot of Mouse IgG F(c) Fragment Biotin Conjugated. Row: Mouse IgG F(c) Biotin Conjugate [1.5mg/mL]. Column Dilutions: 100ng, 33.33ng, 11.11ng, 3.70ng, 1.23ng. Primary Antibody: None. Secondary Antibody: Streptavidin Peroxidase (p/n S000-03) at 1:40,000 for 30mins at RT. Block: BlockOut buffer (p/n MB-073). Exposure: 2 sec. |
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SDS-PAGE of Mouse IgG Whole Molecule Rhodamine Conjugated (p/n 010-0002). MW: 5 µL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Mouse IgG Whole Molecule Rhodamine Conjugated (p/n 010-0002). Lane 2: Reduced Mouse F(c) Fragment (p/n 010-0103). Lane 3: Reduced Mouse F(ab) Fragment (p/n 010-0105). Lane 4: Mouse IgM Kappa Myeloma Protein (p/n 010-001-033). Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa, F(c) at 25 kDa, F(ab) at 25 kDa, IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher. |
