ELISA: 1:2000 - 1:20,000, FLISA: User Optimized, Flow Cytometry: 1:1000-1:5000, IF Microscopy: User Optimized
Application Notes:
Mouse IgG2b kappa isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Dot Blot Results of Mouse IgG2b Isotype Control Allophycocyanin Conjugated. Mouse IgG2b isotype APC Conj (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Exposure: 1 second with DyLight 650 filter.
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